OVERVIEW

OVERVIEW

OPENING SCREEN

INFOBAR
The tabs at the top of the screen allow you to access the Introduction, the Notebook, Assignments, Glossary, and Help. This Infobar will be available on all screens of TranslationLab.


LABORATORY
It is important to review the different equipment portrayed in the laboratory in order to understand the mechanics of the process of translation. Roll the mouse over each piece of equiment illustrated on the left until you see a red box outlined around the equipment. Click on the outlined area for a description of each piece of equipment and its function. The pieces of equipment you will be introduced to are a protein sequencer/scintillation counter, nucleotide bottles, translation mix, RNA synthesizer, and the Notebook.

MAKING RNA

In order to produce an RNA chain to translate, you will use the RNA synthesizer shown in this screen. The four bottles on the left represent possible input nucleotides. The bottle to the right will be filled with synthesized RNA once the machine is activated. On the far right a notebook is presented for your use. See Notebook for more details on using the notebook.

RNA SCREEN


RNA SYNTHESIZER CONTROLS

CONTROL BUTTONS

TRANSLATING RNA

TRANSLATION SCREEN

Next, your RNA sequence goes to the translation mix and then to the protein sequencer. A magnified view of the readout panel on the sequencer will tell you which amino acids your choices produced, OR it will tell you that no protein was produced.

NOTEBOOK

As you run experiments, form hypotheses and test them, you should keep notes on your theories and findings in the TranslationLab notebook.