In FlyLab you studied modes of inheritance and chromosome maps in Drosophila melanogaster. In this laboratory you will have the opportunity to use pedigree analysis to simulate the inheritance of genes for human genetic disorders, and use RFLP analysis to study recombination in humans. Combining RFLP analysis with pedigree analysis will enable you to learn about, and understand, how the location of a gene can be assigned to a chromosome and you will pinpoint the location of a gene on a chromosome by using recombination data to generate a genetic map of that chromosome.
For your ease in completing each assignment, the background text relevant to the experiment that you will perform is blue, instructions for each assignment are indicated by plain text, and questions or activities that you will be asked to provide answers for are indicated by bold text.
The purpose of this laboratory is to allow the student to:
Before You Begin: Prerequisites
Before beginning PedigreeLab you should be familiar with the following concepts: