OVERVIEW

 

 

GENERAL CONTROLS

INFOBAR
The tabs at the top of the screen allow you to access the Introduction, Notebook, Assignments, Glossary, and Help. These tabs will lead you to information and tools that are outside the applet itself.

 

RUNNING EXPERIMENTS

 

There are several different screens in EnzymeLab. You will need to use each screen to complete your experiments. A description and instructions for using each screen appears below.


Laboratory

It is important to review the different equipment portrayed in the laboratory in order to understand how parameters of enzyme kinetics are measured and calculated in the laboratory. Roll the mouse over each piece of equipment illustrated above until you see a blue box outlined around the equipment. Click on the outlined area for a description of each piece of equipment and its function.

 


Choose Options

 

Allows you to add substrate, invertase, and in some reactions, inhibitors, to a test tube to measure the rate of invertase activity. Move the substrate[S] slider back and forth to change the number in the text box to the right. The inhibitor [I] slider will become enabled only if you select an inhibitor. This screen also allows you to select different temperatures and buffer conditions by clicking on the arrows next to the temperature and buffer boxes. Click on the "Add Enzyme and Go!" button to start your experiment.


Measure Curve

 

Allows you to measure the slope of your curve. Click on the graph to move the red line until its slope matches the slope of the initial part of the black curve. When you have moved the red line close to the correct place, it will snap into the correct position and change to blue. Click on "Record Data" to record your results. Click on "Clear Experiment" to start a new experiment.


Plot Data

 

 

Title: Create a title for the plot by typing a name in the text field.

Plot: You can create several separate plots. Use the up and down arrows to choose a number to identify each plot. Plots appear as new tabs in this window.

Data for Curve: You can fit separate curves to different groups of data points that you want to compare on the same graph. Use the up and down arrows to choose a number to identify different groups of data on the same plot.

Plot Type: Use the pull down menu to select from six different plot types.

Symbol: Choose a symbol (square, circle, diamond, cross) to identify the plot points for

different groups of data.

Color: Choose a color to identify the plot points and curves for different groups of data.

Plot Selected Data: Adds the selected group of data to the plot.

Add to Notes: Saves the selected data in your notebook.

Delete Curve: Removes group of data identified in the Data for Curve field from the plot.

Delete Selected: Removes highlighted rows in the table.

 


NOTEBOOK

As you run experiments, form hypotheses, and test them, you should keep notes on your hypotheses and findings in the EnzymeLab Notebook.

Main Window: The main section of the Notebook is an editable text box to which you can add and modify text at any time. The contents of the Notebook are not saved between uses of Lab; therefore, it is suggested that you "Export" and print or save the Notebook contents if you would like a permanent record of your work.

Control Buttons: You will see a row of control buttons at the bottom of the notebook window. These include Export, and Undo Editing.

Export Notes: The current contents of the notebook are exported to a temporary HTML file. This file is then opened in your browser. Exporting your notes allows you to print them when you are done with your assignment.

NOTE: extremely long notes will cause machines to hang when trying to export. If you are running many experiments it is best to print your notes and clear the notebook occasionally.

Undo Editing: Any changes you have made to the notebook since it was last opened will be removed, restore it to its state when it was opened.